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The PCR products were digested with Nco I and Bam HI and ligated into the pKM260 expression vector (EUROSCARF, Frankfurt, Germany), which resulted in the N-terminal addition of a hexahistidine tag and linker with the sequence MHHHHHHASENLYFQGAM
Xu M, Zhang M, Wu J, Wang J, Wu H, Xu X (2025) Esculin promotes skin wound healing in mice and regulates the Wnt/-catenin signaling pathway
It inhibits the activation of NF-B, a transcription factor responsible for the expression of numerous pro-inflammatory mediators
Modeling psychomotor retardation using iPSCs from MCT8-deficient patients indicates a prominent role for the blood-brain barrier
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