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Furthermore, we applied Annexin-V/PI flow cytometry to differentiate and quantitatively determine viable cells [LL quadrant: Annexin V (), PI ()], early apoptotic cells (UL quadrant, Annexin V (+), PI ()], late apoptotic cells [UR quadrant, Annexin V (+), PI (+)], and necrotic cells [LR quadrant, Annexin V (), PI (+)]