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The cells were cultured in DMEM-F12 (HyClone) supplemented with 10% FBS and 1% penicillin/streptomycin at 37 C in a humidified atmosphere of 5% CO 2

The successful transmission of neural signals from one cell to another in animals relies on the mutual conversion of chemical signals (neurotransmitters) and electrical signals, where ion fluxes are induced by neurotransmitters and the release of neurotransmitters is dependent on electrical signals 1,2,42

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Zhang L, Yao G, Mao Z, Song M, Zhao R, Zhang X, Chen C, Zhang H, Liu Y, Wang G, Li F, Wu X

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