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doi: 10.1038/nrgastro.2013.149 69

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The experimental setup involved exposure of dermal fibroblasts to various combinations of GHK-Cu and HA, measuring the synthesis of collagen types I, IV, and VII

A unit of 40 g of protein lysate was incubated with SDS sample buffer for 5 min at 90 C, resolved on a 10% polyacrylamide gel and electroblotted on a polyvinylidene difluoride membrane

Due to being more readily absorbed compared to traditional L-Carnitine, ALCAR has also been shown to improve glucose tolerance and insulin sensitivity along with serum lipids

Statistical significance was determined by multiple two-tailed unpaired t-tests with correction for multiple comparisons using the Holmdk method