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20 Detection Methods There are numerous methods for detecting glutathione, with commonly used ones including the iodometric method, the DTNB (Ellman's reagent) method, the sodium nitroprusside method, the fluorometric method, the alloxan method, and high-performance liquid chromatography (HPLC)
For LC-MS analysis, samples were thawed on ice-water bath, then 40 L of whole blood was injected into 450 L cold extraction solvent comprising of mixture of Acetonitrile:Methanol:Milli-Q water in ratio 40:40:20 according to protocol described in Presterud et al 35
This cytoprotective effect involves upregulation of antioxidant defense systems and stabilization of mitochondrial function, with Dihexa-treated cells maintaining healthier mitochondrial membrane potentials under stress conditions
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Blood pressure medications (ACE inhibitors, ARBs, beta blockers, calcium channel blockers, diuretics) work normally on semaglutide
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