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The specific biomarkers focused on were adiponectin, leptin, IL-6, TNF-, plasminogen activator inhibitor-1 (PAI-1), brain natriuretic peptide (BNP), and high-sensitivity C-reactive protein (hs-CRP)

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A study that compared 24 obese patients with MC-4R deficiency to 80 healthy controls (40 obese and 40 lean) found that those with MC-4R mutations consumed significantly more high-fat meals

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Three of the seven days are smoothie-based for low-appetite flexibility