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The absorbance was measured at 450 nm with a Synergy H1 Multi-Mode Microplate Reader (BioTek, USA).When running an ELISA, the values of the samples are assigned in relation to the standard curve

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Symptoms include drowsiness, confusion, blurred vision, numbness or tingling in your mouth, trouble speaking, muscle weakness, clumsy or jerky movements, seizure (convulsions), or loss of consciousness