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This subclinical deficiency phase is associated with low or marginal vitamin B12 concentrations

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The 2017 Neelakantan paper in Biochemistry characterised a fluorescent assay for direct real-time monitoring of NNMT enzymatic activity, providing the analytical foundation for inhibitor screening including 5-Amino-1MQ (PMID 28121423)

Route of administration: IM, IV or SC as directed by veterinarian