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We then capped any unreacted cysteine thiol and cysteine persulfide using -(4-hydroxyphenyl)ethyl iodoacetamide (HPE-IAM) and analyzed the intact mass product distribution using ESI-MS

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At 10 mg/mL : The top two rungs exceed a single 100-unit barrel, so at 12.5 and 15 mg the volume must be split across two injections or the vial reconstituted at a higher concentration with less water