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Next, to analyse the functional impact of SPHKAP downregulation on islet function, we employed a lentiviral shRNA approach to knock down SPHKAP on intact islets, as most functional outputs from primary islets cannot be optimally recapitulated with dispersed islet cells due to the loss of important intra-islet cell-cell interactions

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Pichard, C., Roulet, M., Schutz, Y., Rossle, C., Chiolero, R., Temler, E., Schindler, C., Zurlo, F., Furst, P., and Jequier, E

It was reassuring to know someone was there to keep an eye on things