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Troubleshooting timing-related problems When copper peptide results aren't meeting expectations, timing issues are often part of the problem

The first step is the unusual coupling of the gamma-carboxylic acid of glutamic acid to cysteine by the enzyme gamma-glutamylcysteine synthase to form gamma-glutamylcysteine

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The radioactive counts of the bound antigen-antibody complexes and the free antigen are used to calculate the concentration of the non-labeled antigen

Nature 566 , 115119 (2019)