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LC-MS/MS data quality evaluation and statistical analysis were done using software Perseus v.1.6.15.0 Untargeted metabolomics Cells were seeded in a 12-well plate with an optimized cell confluency ( 1500 quantifiable peaks of putatively annotated metabolites
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The resulting microsomal pellet was resuspended in storage buffer (0.1 M potassium phosphate, pH 7.4, 20% glycerol, 1 mM EDTA/Dithiothreitol) and kept at -80 C until further use in enzyme activity assays
The optional Precision Peptide Genetic Test analyzes 17 Longevity insights including FOXO3 the most-studied longevity gene, linked to centenarian populations and cellular repair capacity with crossover into Energy Metabolism, Tissue Repair, and Cognition pathways
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