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Overexpressed proteins were purified on a Ni-NTA column (Cytiva) with an elution buffer containing 50 mM NaH 2 PO 4 , 300 mM NaCl, and 300 mM imidazole

Membrane expansion alleviates endoplasmic reticulum stress independently of the unfolded protein response

Patients experiencing such symptoms should stop taking Ozempic immediately and seek urgent medical attention

This graduated approach minimizes side effects while progressively increasing therapeutic benefit

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