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Preparing The B12 Injection Site Before administering the injection, it's essential to prepare the injection site properly to minimize the risk of infection and ensure optimal absorption of the B12 solution

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You draw doses with an insulin syringe

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After fixation, the cells were washed with PBS and permeabilized with 0.1% Triton X-100 (Daejung 8566-4405) in PBS for 10 min at room temperature

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