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Glutamate and glycine, the other two precursors, are generally not limiting
Ideal BAC Water (mL) = Vial Size (mg) Target Volume (mL)Desired Dose (mg) Examples for Tricky Reconstitutions 400 mcg (0.4 mg) dose in a 10 mg vial: Target 10 units (0.1 mL)
(2003) 133(11 Suppl 2):3927S3931S
If bubbles appear, push the fluid back into the vial and redraw more slowly
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This combination leads to more consistent outcomes
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