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Samples were then left for 20 min on RT, after which they were diluted 1 + 4 with ammonium bicarbonate (25 mM, pH = 8.5) and then digested overnight with trypsin (1 g of trypsin per sample)
Key aspects to monitor include the patients weight and Body Mass Index (BMI)
If persistent, delay dose escalation
The preclinical record in animals is large
Curiously, they also seemed to suppress appetite, and some people taking them lost a modest amount of weight
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