This isn't because 1:1 is mechanistically optimal in some provable way it's because researchers studying combinations of these compounds chose 1:1 as the default and the literature accumulated around that choice
Let's discover some broad recommendations for doses according to frequent medical uses
In such cases, proportionately more B12 is bound to holoTC leading to elevated serum holoTC levels
All are supplied in lyophilized form with full COA documentation

Why bacteriostatic water is the standard for peptide reconstitution In laboratory practice, the choice of solvent is governed by several requirements at once, and bacteriostatic water meets them all: Neutrality toward the peptide , it has a near-neutral pH and contains no salts or ions that could react with the peptide molecule or affect its stability Compatibility , it works with the vast majority of common research peptides without causing precipitation, aggregation, or degradation Extended solution shelf life , thanks to the preservative, the reconstituted solution lasts substantially longer with proper storage than with sterile water Safety of repeated withdrawal , a single vial serves for multiple withdrawals over days, which is essential when working with small doses Availability and standardization , it is an established, well-documented solvent familiar to everyone who works with peptides When something else is used For completeness, it should be added that bacteriostatic water is not the only possible solvent, merely the most common

Effects on specific populations including the elderly, those with metabolic disease, and women have not been specifically investigated