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Several factors can affect results: Age and sex Pregnancy Fasting status Hydration Recent exercise Acute illness Supplements and other medications Anemia Kidney function Weight and body-composition changes Laboratory methodology An abnormal result does not always indicate disease, and a normal result does not always exclude a clinically important problem

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A.GarciaE.NavisG.et al (2021)

But the shift can feel disorienting, especially if you're used to planning meals around consistent hunger

Such as, you can detect 80 samples without duplication or 40 samples

Categorical variables were expressed as frequencies and percentages