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4 cells were seeded in DMEM + 10% FBS in white-walled 96-well plates (Costar) and allowed to attach for 48 hours at 5% CO 2 /air, 37C

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Notably, TJ length was reduced in the same study, and claudin-3 knockout mice exhibited decreased infarct size and reduced edema formation

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Antigens are recognized, taken up, and internalized by APCs, then loaded and presented on the cell surface via MHC molecules in this process

To sum up, IGF-1 LR3 may boost all the anabolic effects of growth hormone and that is a powerful tool in terms of muscle mass and so on, but it does not directly raise GH levels, so could not be used for growth hormone deficiency