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Fresh stock solutions of NADPH, GSH, GR and GSSCys or HEDS were prepared in assay buffer containing 0.1 M Tris/HCl, 1 mM EDTA, pH 8.0 before each experiment

However, this study examined only the effects of lipotropic substances in the rats diet, not lipotropic injections, and has limitations as an animal study

Women in the control group may have been using non-prescription GLP-1s (which would have diluted the observed effect)

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