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Pre-cycle baseline labs: Telomere length testing (qPCR or Flow-FISH method) 6-sulfatoxymelatonin (first morning urine or 24-hour urine collection) AM cortisol (drawn before 9:00 AM) DHEA-sulfate Complete metabolic panel hsCRP (as an inflammatory marker) Fasting insulin and HbA1c Post-cycle follow-up (6 to 8 weeks after completing a course): Repeat 6-sulfatoxymelatonin Repeat telomere length (though detectable change may require 6 to 12 months) Subjective sleep quality assessment (Pittsburgh Sleep Quality Index) Telomere length testing carries its own limitations

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Reported adverse effects in small human studies include headache, dizziness, and transient blood pressure changes

PMID: 15465785 | DOI: 10.1093/jn/134.10.2783S [6] Heard KJ

Participants were male and female adults aged 18 years who were of non-childbearing potential and met the BMI inclusion criteria of either 30.0 kg/m, or 27.0 kg/m with hypertension or dyslipidemia

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