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They metabolize through different pathways
In this report we demonstrate the efficacy of multiplexing the CellTox Green Cytotoxicity Assay with the GSH/GSSG-Glo Assay to determine both the cytotoxic and oxidative effects of compounds on cultured cells in a single assay plate
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Start at the lower end of the dosing range to assess individual response
Following purification by preparative reversed phase (high-performance liquid chromatography), a sample was injected on analytical HPLC to determine a final purity of 97.7% by HPLC peak area
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