It was developed to provide a chemically well-characterised molecule suitable for controlled investigation of short peptide signalling in cellular and molecular research systems
Enzymatic Reaction Conditions The substrate solution used for the NsPCS reaction was prepared as follows: 28.7 mg of tris (2-carboxyethyl) phosphine hydrochloride (TCEP) (Tokyo Chemical Industry) and 3.0733 g of GSH (Nacalai Tesque) were weighed in a 100 mL volumetric flask, 20 mL of 1000 mM potassium phosphate buffer (pH 8.0) was added, and the solution was gently shaken
This is due to their potential for some serious side effects, such as an allergy, although they occur very rarely
Authors : Wenzhuo Li, Qingtong Zhou, Zhaotong Cong, Qingning Yuan, Wenxin Li, Fenghui Zhao, H
doi: 10.2147/DMSO.S216791 19
$500 if purchased separately, saving $106)