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After reconstitution with bacteriostatic water, refrigerate and use within your protocols window, observing good laboratory practice

Cells were washed with PBS plus 0.02% Triton X-100 (Sigma-Aldrich, Dorset, UK) 3 times for 5 min each and blocked with 10% donkey serum (Sigma-Aldrich, Dorset, UK) for 30 min

After 15 min of exendin 2029 or exendin-4 administration, a glucose solution (2 g/kg) was intraperitoneally injected (at 0 min

The HIF-/ dimer binds with the transcriptional coactivator p300/CBP and hypoxia response element to induce the expression of the HIF target gene located in the promoter region [22, 23]

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Cycling protocols (8 weeks on, 4 weeks off) are designed around this adaptation curve