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Apple embryonic axes (25 mg) were homogenised in 50 mM K-phosphate buffer (pH 7.5), with 0.1% triton X-100, 5 mM DTT (Sigma, D0632), 5% (w/v) glycerol, 0.1% protease inhibitor cocktail (Sigma, P9599) and 2% (w/v) PVPP on ice

Store lyophilized GHK-Cu at -20C, protected from light

Practical Considerations for Researchers The decision between bacteriostatic and sterile water should be based on how the peptide will be used

It binds to and regulates the translation of ACSL4 mRNA in an m 6 A modification-dependent manner, promoting the activation and ferroptosis of HSCs

Cell cytoplasm constitutes the most abundant pool of ascorbate, while to some extent it is also transported across plasma membrane (usually 5%) to the apoplast (Barnes et al., 2011