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The lysed tissue was centrifuged (13000 rpm, 3 min, 4 C) and the supernatant was transferred to a clean Eppendorf tube (low-bind protein) prior to protein concentration determination with the DC Protein Assay (Bio-Rad) according to manufacturers instruction

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Because orforglipron is taken once a day in pill form, it may be easier for patients to stay consistent with treatment