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Como siguiente paso, Ramich y Pfeiffer tienen la intencin de hacer un seguimiento de sus hallazgos sobre los mecanismos involucrados y as obtener nuevos conocimientos sobre el modo de accin de las estrategias de intervencin diettica especficas
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Western immunoblot analyses For the preparation of total liver homogenates, frozen liver pieces were homogenized in RIPA buffer (150 mM NaCl, 1% TritonX-100, 0.25% sodium deoxycholate, 0.1% SDS, 50 mM Tris, 1 mM EDTA, 1 mM PMSF, protease inhibitor cocktail, pH 7.4) on ice with a tissue tearor (BioSpec Products, Bartlesville)
10.1089/ars.2016.6866 82 YuF.YuC.LiF.ZuoY.WangY.YaoL.et al (2021)
Although the therapeutic dose of liraglutide appeared to enhance bone material properties, the most significant improvements in BMD and microarchitecture were noted at doses much higher than those used for weight loss in PwO
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