Samples were washed and subsequently incubated with secondary antibody for 45 min at RT (Table 3) Sections were counterstained either with the diaminobenzidine substrate kit (Nacalai, USA) followed by hematoxylin, or sections were stained with 6-diamidino-2-phenylindole (DAPI, Thermo Fisher, USA) for visualization of cell nuclei as previously described
3 (download XLSX Source data for Fig
Evidence suggests that vitamin D 3 generally leads to a greater increase in total serum 25(OH)D concentrations than vitamin D 2 , even when analyses are restricted to daily-dose supplementation studies
It is a research-grade compound supplied strictly for research use only
While all three have some similarities in their mechanisms of action, potency, and potential outcomes from a clinical perspective, they also have some significant differences in terms of selectivity and side effects
In fact, a plethora of studies clearly indicate that neuroinflammation is important to the progressive brain dysfunction seen in demented persons, who exhibit an array of cognitive deficits including non-spatial memory impairment 72,73