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Rats receiving vagotomy do not reduce food intake in response to peripherally injected GLP-1 indicating that vagal afferent nerves are needed to relay peripheral GLP-1 signals to higher brain regions to regulate feeding (Abbott et al., Phox2b-Cre Glp1r flox/flox ) (Sisley et al., Phox2b-Cre Glp1r flox/flox mice fed a HFD reduced food intake, body weight, and adiposity suggesting that the PNS is not required for liraglutide to mediate its effects on energy metabolism

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