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Swirling the vial slowly afterward never shaking it supports full dissolution while protecting the peptides molecular structure, since vigorous agitation can degrade sensitive peptide bonds
To investigate the depletion of O 2 within the cells, cancer cells in a 1% O 2 atmosphere were treated with Ru(II)-OH, NP2, or NP4, and the O 2 concentration was assessed using the hypoxia probe pimonidazole hydrochloride
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The percentage figure appears on the Certificate of Analysis next to the HPLC purity and LC-MS identity results, making it straightforward to confirm both the peptide and the metal coordination before any cell-based work begins
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