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After stabilized in Krebs buffer for 30 min, aortic rings were depolarized with potassium chloride (60 mmol/L) for evaluation of their maximal contraction

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Sex steroid measurements In order to accurately perform a comprehensive analysis of sex steroid profile in both PCOS models, specific protocols of LC-MS/MS were applied as described elsewhere 78

Advancing brain barriers RNA sequencing: guidelines from experimental design to publication