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Tilt the vial at a 45 angle to allow the solvent to gently run down the interior wall
Biosynthesis of melatonin: enzymic conversion of serotonin to N-acetylserotonin
Comparable fermentation profiles were observed at 25 C and 30 C, indicating minimal differences in metabolic activity under these conditions (data not shown)
The pituitary gland and hypothalamus were collected and frozen for analysis of mRNA expression of GH, GHRH, GHSR, SST, and SSTR by real-time polymerase chain reaction (RT-PCR) and protein expression by Western immunoblotting
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