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Palao M, Fernndez-Daz E, Gracia-Gil J et al (2020) Multiple sclerosis following SARS-CoV-2 infection
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The fractions were lysed with the modified NETN buffer (50 mM Tris, pH 7.4, 150 mM NaCl, 0.5% Nonidet P-40, 100 U/mL MNase, 100 U/ml Benzonase) plus protease inhibitor at 37 C for 20 min
Of note, hemolysis is a fundamental feature of SCD and resulting excess hemin exposure 32 while hypoxia has been shown to induce the expression of fetal hemoglobin 33,34 , and promote the transition from expansion to differentiation during stress erythropoiesis 35
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