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The apoptosis flow cytometry kit, caspase-3 activity assay kit, and mitochondrial complex I detection kit were purchased from Beyotime Biotechnology (China) and GenScript Corporation.Trypsin-EDTA digestion solution (FL20011) were purchased from Bioland Biotechnology Co

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The DIRECT [Diabetes Remission Clinical Trial] trial showed that a weight loss of only 5kg had the ability to prevent development of T2DM, and those that lost 10kg were predominantly still in the non-diabetes range two years later. Ruszala said that her main question about the trial would be about the gastrointestinal side effects experienced by the participants