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It directly upregulates COL1A1, COL1A2, and COL3A1 at the mRNA level in human skin fibroblasts (quantified by qRT-PCR and northern blot), increases collagen protein synthesis (measured by [H]-proline incorporation into hydroxyproline-containing collagen peptides by Sircol assay), and promotes lysyl oxidase activity (copper-dependent cross-linking collagen fibrils for tensile strength)
If the ER response is overwhelmed, substantial amounts of calcium are released into the cytoplasm, leading to apoptosis
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Identifying key molecules and pathways involved in this process can inform strategies to enhance ACL resilience and repair
& Busse, R
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