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Definition: CAIs are a class of diuretics that inhibit the enzyme carbonic anhydrase found primarily in the proximal convoluted tubule of the nephron, not first-line diuretics for hypertension or heart failure due to their weak natriuretic effect Mechanism of Action: They block carbonic anhydrase, which reduces the reabsorption of bicarbonate (HCO), sodium (Na), and water, leading to increased diuresis and alkaline urine