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Protein concentration was determined using the Pierce bicinchoninic acid assay (Life Technologies #23225) and 40 g of protein were loaded per lane on a 412% Bis-Tris or 38% Tris-Acetate gel (Life Technologies #NP0323 and #EA03785)

Additionally, insufficient intake of vitamins and minerals (like vitamin C, selenium, and magnesium) that support synthesis, can further reduce levels

PMCID: PMC4064320

They not only help with skin whitening but also improve liver health, immunity, and overall wellness

Determination of cellular redox status by stable isotope dilution liquid chromatography/mass spectrometry analysis of glutathione and glutathione disulfide

These agents scavenge free radicals, regenerate other antioxidants, and support mitochondrial function