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Immunoblotting Cells or tissues were lysed with RIPA lysis buffer (150 mM NaCl, 100 mM Tris, 5 mM EDTA, and 1% Triton-100) supplemented with 0.1 mmol/L PMSF (Beyotime, Shanghai, China, #ST505) and 1 mmol/L protease inhibitor cocktail (MCE, Monmouth Junction, NJ, #HY-K0010)

Its not specific to a particular medication, like Ozempic, Wegovy, Mounjaro or Zepbound

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Secondly, macrophages obtained were stimulated in vitro with growth factors and cytokines, which might not reflect how TAMs switch in vivo

Focus remains on tolerating the medication and establishing the routine

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