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Beads were pelleted, washed, PNGase F-treated (G5166, Sigma) (if applicable), and proteins eluted in SDS sample buffer/2.5% mercaptoethanol

Emerging evidence highlights the critical role of glutamine as an alternative carbon source for bioenergetics and anabolic biosynthesis in addition to glucose [21,22,23]

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The central concern is how to optimize tissue perfusion while minimizing the risk of iatrogenic fluid overload