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This effect was not seen when the DHHS-mutant of zDHHC5 was used, suggesting that GCP16/Golga7 is a substrate for zDHHC5 (Supplementary Fig
As control, we used the GFP-FM 4 -hGH construct that aggregates in the ER lumen and is secreted via Golgi-mediated transport after chemical dissociation 29
Therefore, the weaker activity of the Golgi-associated zDHHC3 and 7 toward Go-Nt 31 predicts an increase in its PM accumulation upon time, in agreement with the experimental findings (Fig
Its a resounding yes
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The peptide tells cells what to do
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