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After 2 h of incubation, viability was assessed by measuring the fluorescence using a 540 20 nm excitation filter and a 590 20 nm emission filter on a Spark microplate reader (Tecan)

Heres the simple, no-nonsense version: Initial results: 46 sessions Visible glow: Week 3 onward Peak brightness: Around 2 months Duration of results: 36 months Maintenance: Once every 46 weeks Glutathione works beautifully, but its a treatment , not a permanent switch

10.1073/pnas.0403412101 Proc

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BPC-157 is also classified by WADA as a prohibited substance for competitive athletes, which is a regulatory consideration separate from safety