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While we did not specifically evaluate the M1/M2 macrophage phenotype in our study cohorts, the observed pro-inflammatory immune profile suggests an activation of M1-type macrophages in patients with LC
BMC complementary Altern
The beads were washed once with 10 ml of lysis buffer, followed by three washes with 10 ml of wash buffer (50 mM Tris, pH 7.5, 300 mM NaCl, 20 mM imidazole, 1 mM DTT)
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