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This dose was chosen based on a previous dose-escalation study and subsequent work demonstrating its efficacy to drive weight loss in a short period of time 8

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It used a ShapiroWilk test to assess normality and an F-test to assess homoscedasticity, and attempted log-transformation to resolve any lack thereof 40

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thus, analysis of pooled unclassified genera has severe limitations

Paired-end FASTQ files were then run through the DADA2 pipeline (v1.8) to generate an amplicon sequence variant (ASV) table and assign taxonomy