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Biofilm fluorescence intensity was then determined at Ex 544 nm and Em 590 nm (Tecan infinite M1000 pro, Australia)

Swab the rubber stopper with 70% isopropyl alcohol and allow it to dry before needle insertion

Cologne Center for Genomics (CCG) supported nextgeneration sequencing

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Experiments are designed to quantify its effects on collagen I and III synthesis (via qPCR or Western blot), myofibroblast differentiation (by measuring -smooth muscle actin expression), and the critical MMP/TIMP balance

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