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10.1016/j.cbi.2018.05.002 57 RaslanM.Abdel RahmanR.FayedH.OgalyH.FikryR

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After the corresponding treatment, cells were fixed with cold methanol for 5 min, blocked with 1% BSA in phosphate-buffered solution (PBS) for 1 h, and then incubated overnight at 4 C with anti-collagen 1 type I antibody (Santa Cruz), anti-collagen IV (20421, Novotec, Lyon, France), collagen 1 type III (sc271249, Santa Cruz), anti-fibronectin (sc8422, Santa Cruz) and LOX (ab174316, Abcam), followed by the corresponding fluorescent secondary antibodies

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