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Mixing Instructions

GSH Quantification Glutathione and GSSG were measured in lysates from cells in 6-well plates as previously described ( ROS Quantification by Spin-Trapping and EPR Spectroscopy Cells (5 10 6 /0.1 ml) were stimulated for 2 h with LPS, then incubated with 50 mM 5-tert-butoxycarbonyl-5-methyl-1-pyrroline-N-oxide (BMPO), synthetized as described previously ( Cell Viability Assay Cells were seeded in 96-well plates at 25,000/well in complete medium

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RSL3 stimulation elevated C3, IL-1, and TNF- levels in mixed glial cultures, while the ferroptosis inhibitor liproxstatin-1 effectively attenuated RSL3-induced neurotoxicity, supporting a role for ferroptotic stress in promoting neurotoxic glial activation

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