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Binding affinity of the GSSSG/LASSS-pretreated HGF ( bars 7 and 11 ) to c-met was significantly higher than that of nitrated HGF ( bar 3 , peroxynitrite-treated at 1:2000) and reached approximately 80% of the full activity of the positive controls exposed to untreated HGF ( bars 2 and 2 )