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Reduction and Alkylation : Disulfide bonds (if present in other peptides) were reduced with DTT and alkylated with iodoacetamide (not necessary in Semaglutide but part of standard workflow)
5B and S6E )
Time is cumulative
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MIG-6L(WT):mNG was present within the DTC and the gonadal basement membrane, consistent with its reported DTC-specific expression and secretion throughout gonad development (Supplementary Fig
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