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WB assay Equal amounts of protein were separated by SDS-PAGE and then transferred onto nitrocellulose membranes

The mass spectrometer was operated in positive electrospray ionization mode at a collision energy of 12 V, spray voltage of 4000 V, cone and probe temperature of 350C, cone gas flow rate of 20 L min 1 , probe gas flow rate of 40 L min 1 , and nebulizer gas flow of 60 L min 1

Clear solution This protocol yields a clear solution of 5 mg/mL (saturation unknown)

This has implications in blast induced TBI in a battle field setting

Bradshaw, J

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