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Briefly, the top and bottom strand oligonucleotides were annealed and ligated into the Gateway-based pENTR/U6 vector (Thermofisher) and sequence confirmed

Protects against cell damage: Glutathione may help to protect against cell damage by neutralizing free radicals

Therefore, in order to contextualize the complexity of the metabolic network and the impact of Gclc on it, we analyzed our transcriptomic data using Compass, a flux balance analysis-based approach that allows the characterization and interpretation of metabolic diversity at the single cell level 70,71

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